Protoplast Regeneration from Normal and Bromodeoxyuridine-Resistant Sycamore Callus

Callus culture and regeneration.

Clostridium acetobutylicum Protoplast Formation and Regeneration.

Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described.

Protoplast Formation and Regeneration in Acetobacter Pasteurianus

Method for production and regeneration of Acetobacter pasterianus protoplasts is described. The protoplasts were obtained by treatment of cells with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with NaCl (0.5M) as ...

Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid

Embryogenic calli were induced from longitudinally bisected segments of protocorm-like bodies (PLBs) of Cymbidium Twilight Moon ‘Day Light’, a hybrid orchid, on modified Vacin and Went medium [Bot. Gaz. 110 (1949) 605] supplemented with 1-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ) within 1 mont...

Callus Induction and Shoot Regeneration from Epicotyl Explants of Ethnomedicinally Important Caesalpinia bonduc (L.) Roxb

An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS mediumsupplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calliformed were excised and subcultured on MS medium fortified with 1 mg/l...